Isoflurane ameliorates oxygen-glucose deprivation-induced cardiomyocyte injury through SIRT6/DNMT1 pathway

The incidence of cardiovascular diseases is on the rise in world, which poses a significant threat to human health. Myocardial ischemia can cause heart disease. Therefore, it necessary avoid myocardial hypoxia/reoxygenation (H/R) injury attenuate risk present study focuses protective effect isoflurane H/R-induced cell through Sirtuin 6 (SIRT6)/DNA (cytosine-5)-methyltransferase 1 (DNMT1) pathway. Quantitative reverse transcription PCR (RT‑qPCR) and Western blot analysis were used measure protein levels mRNA expression H9c2 cells. Cell Counting Kit‑8 assays (CCK8 assay) was determine viability. pro-inflammatory molecule assessed using commercial Enzyme-linked immunosorbent assay (ELISA) Kits. ratio cellular apoptosis determined by flow cytometry. contents Lactate dehydrogenase (LDH), Cardiac Troponin I (cTnI), Creatine Kinase MB (CK-MB) detected colorimetric assays. This shows that Isoflurane reduces DNMT1 activating SIRT6 oxygen-glucose deprivation (OGD)-induced H/R injury. damage cardiomyocyte decreased after treatment under OGD exposure condition. In addition, ameliorates OGD-induced inflammatory responses via interaction with SIRT6/DNMT1 Taken together, this suggested process provided new mechanism action for